Publications

2021

• Sui, Y., Mazzucchi, L., Acharya, P., Xu, Y., Morgan, G., Harvey, P.J. (2021) A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths. Foods, 10, 2824. https://doi.org/10.3390/foods10112824
• Xu, Y.; Harvey, P.J. (2021) Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina. Mar. Drugs , 19, 595. https://doi.org/10.3390/md19110595
• Sui, Y, Harvey, PJ. (2021). Effect of light intensity and wavelength on biomass growth and protein and amino acid composition of Dunaliella salina Foods 10 (5): 1018. https://doi.org/10.3390/foods10051018

2020

• Sui, Yixing, Jiang, Yu, Moretti, Michele and Vlaeminck, Siegfried E. (2020) Harvesting time and biomass composition affect the economics of microalgae production. Journal of Cleaner Production, 259:120782. ISSN 0959-6526 (doi:https://doi.org/10.1016/j.jclepro.2020.120782)
• Xu, Yanan  and Harvey, Patricia J.  (2020) Phytoene and phytofluene overproduction by Dunaliella salina using the mitosis inhibitor chlorpropham. Algal Research, 52:102126. ISSN 2211-9264 (doi:https://doi.org/10.1016/j.algal.2020.102126)
• Harvey, Patricia J.  and Ben-Amotz, Ami (2020) Towards a sustainable Dunaliella salina microalgal biorefinery for 9-cis β-carotene production. Algal Research, 50:102002. ISSN 2211-9264 (Online) (doi:https://doi.org/10.1016/j.algal.2020.102002)
• Maneein, Supattra, Milledge, John J. , Harvey, Patricia  and Nielsen, Birthe  (2020) Methane production from Sargassum muticum: effects of seasonality and of freshwater washes. Energy and Built Environment. ISSN 2666-1233 (In Press) (doi:https://doi.org/10.1016/j.enbenv.2020.06.011)
• Mazzucchi, Laura, Xu, Yanan  and Harvey, Patricia  (2020) Stereoisomers of colourless carotenoids from the marine microalga dunaliella salina. Molecules, 25 (8):1880. pp. 1-15. ISSN 1420-3049 (Online) (doi:https://doi.org/10.3390/molecules25081880)
• Kottmann JS, Jørgensen MGP, Bertolini F, Loh A, Tomkiewicz J (2020) Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel. PLoS ONE 15(7): e0235617. https://doi.org/10.1371/journal.pone.0235617
• Milledge, J.J.; Maneein, S.; Arribas López, E.; Bartlett, D. (2020) Sargassum Inundations in Turks and Caicos: Methane Potential and Proximate, Ultimate, Lipid, Amino Acid, Metal and Metalloid Analyses. Energies, 13, 1523.
• Nielsen, B.V.; Maneein, S.; Al Farid, M.M.; Milledge, J.J. (2020) The Effects of Halogenated Compounds on the Anaerobic Digestion of Macroalgae. Fermentation, 6, 85
• Roy, U.K.; Nielsen, B.V.; Milledge, J.J. Effect of post-harvest conditions on antioxidant enzyme activity in Dunaliella tertiolecta biomass. Biocatalysis and Agricultural Biotechnology 2020, 27, 101661.

2019

• Xu, Yanan  and Harvey, Patricia  (2019) Red light Control of β-Carotene isomerisation to 9-cis β-carotene and carotenoid accumulation in Dunaliella salina. Antioxidants, 29 (5):2019050218. ISSN 2076-3921 (Online) (doi:https://doi.org/10.3390/antiox8050148)
• Weinrich, Tobias, Xu, Yanan , Wosu, Chiziezi, Harvey, Patricia  and Jeffery, Glen (2019) Mitochondrial function, mobility and lifespan are improved in Drosophila melanogaster by extracts of 9-cis-β-Carotene from Dunaliella salina. Marine Drugs, 17 (5). pp. 279-288. ISSN 1660-3397 (Online) (doi:https://doi.org/10.3390/md17050279)
• Xu, Yanan  and Harvey, Patricia J.  (2019) Carotenoid production by Dunaliella salina under red light. Antioxidants, 8 (5):123. ISSN 2076-3921 (Online) (doi:https://doi.org/10.3390/antiox8050123)
• Milledge, John J. , Nielsen, Birthe V. , Maneein, Supattra and Harvey, Patricia J.  (2019) A brief review of anaerobic digestion of algae for bioenergy. Energies, 12 (6):1166. ISSN 1996-1073 (Print), 1996-1073 (Online) (doi:https://doi.org/10.3390/en12061166)
• Sui, Yixing and Vlaeminck, Siegfried E.  (2019) Dunaliella microalgae for nutritional protein: an undervalued asset. Trends in Biotechnology, 38 (1). pp. 10-12. ISSN 0167-7799 (Print), 1879-3096 (Online) (doi:https://doi.org/10.1016/j.tibtech.2019.07.011)
• Sui, Yixing, Muys, Maarten, Van de Waal, Dedmer B., D'Adamo, Sarah, Vermeir, Pieter, Fernandes, Tânia V. and Vlaeminck, Siegfried E.  (2019) Enhancement of co-production of nutritional protein and carotenoids in Dunaliella salina using a two-phase cultivation assisted by nitrogen level and light intensity. Bioresource Technology, 287:121398. ISSN 0960-8524 (doi:https://doi.org/10.1016/j.biortech.2019.121398)
• J.J. Miest, S.N. Politis, M. Adamek, J. Tomkiewicz, I.A.E. Butts (2019) Molecular ontogeny of larval immunity in European eel at increasing temperatures, Fish & shellfish immunology Vol. 87, 105-119

2018

• Muys, Maarten, Sui, Yixing, Schwaiger, Barbara, Lesueur, Céline, Vandenheuvel, Dieter, Vermeir, Pieter and Vlaeminck, Siegfried E.  (2018) High variability in nutritional value and safety of commercially available Chlorella and Spirulina biomass indicates the need for smart production strategies. Bioresource Technology, 275. pp. 247-257. ISSN 0960-8524 (doi:https://doi.org/10.1016/j.biortech.2018.12.059)
• Sui, Yixing , Muys, Maarten, Vermeir, Pieter, D'Adamo, Sarah and Vlaeminck, Siegfried E.  (2018) Light regime and growth phase affect the microalgal production of protein quantity and quality with Dunaliella salina. Bioresource Technology, 275. pp. 145-152. ISSN 0960-8524 (doi:https://doi.org/10.1016/j.biortech.2018.12.046)
• Sui, Yixing  and Vlaeminck, Siegfried E  (2018) Effects of salinity, pH and growth phase on the protein productivity by Dunaliella salina. Journal of Chemical Technology & Biotechnology, 94 (4). pp. 1032-1040. ISSN 0268-2575 (Print), 1097-4660 (Online) (doi:https://doi.org/10.1002/jctb.5850)
• Maneein, Supattra, Milledge, John J. , Nielsen, Birthe V.  and Harvey, Patricia J.  (2018) A review of seaweed pre-treatment methods for enhanced biofuel production by anaerobic digestion or fermentation. Fermentation, 4 (4):100. pp. 1-31. ISSN 2311-5637 (Online) (doi:https://doi.org/10.3390/fermentation4040100)
• Milledge, John J. , Nielsen, Birthe V. , Sadek, Manar S. and Harvey, Patricia J.  (2018) Effect of freshwater washing pretreatment on Sargassum muticum as a feedstock for biogas production. Energies, 11 (7):1771. ISSN 1996-1073 (Online) (doi:https://doi.org/10.3390/en11071771)
• Milledge, John J. , Nielsen, Birthe V.  and Harvey, Patricia J.  (2018) The inhibition of anaerobic digestion by model phenolic compounds representative of those from Sargassum muticum. Journal of Applied Phycology, 31 (1). pp. 779-786. ISSN 0921-8971 (Print), 1573-5176 (Online) (doi:https://doi.org/10.1007/s10811-018-1512-4)
• Xu, Yanan , Ibrahim, Iskander M., Wosu, Chiziezi I., Ben-Amotz, Ami and Harvey, Patricia J.  (2018) Potential of new isolates of Dunaliella Salina for natural β-Carotene production. Biology, 7 (1). p. 14. ISSN 2079-7737 (Print), 2079-7737 (Online) (doi:https://doi.org/10.3390/biology7010014)
• Binning, Sandra, Roche, Dominique, Grutter, Alexandra, Colosio, Simona, Sun, Derek, Miest, Joanna Junack and Bshary, Redouan (2018) Cleaner wrasse indirectly affect the cognitive performance of a damselfish through ectoparasite removal. Proceedings of the Royal Society B: Biological Sciences, 285 (1874):20172447. ISSN 0962-8452 (Print), 1471-2954 (Online) (doi:https://doi.org/10.1098/rspb.2017.2447)
• Miest, Joanna J., Politis, Sebastian N., Adamek, Mikolaj, Tomkiewicz, Jonna and Butts, Ian A. E. (2018) Molecular ontogeny of larval immunity in European eel at increasing temperatures. Fish & Shellfish Immunology, 87. pp. 105-119. ISSN 1050-4648 (doi:https://doi.org/10.1016/j.fsi.2018.12.048)

2017

• Politis, Sebastian N., Mazurais, David, Servili, Arianna, Zambonino-Infante, Jose-Luis, Miest, Joanna J. , Sørensen, Sune R., Tomkiewicz, Jonna and Butts, Ian A. E. (2017) Temperature effects on gene expression and morphological development of European Eel, Anguilla anguilla larvae. PLoS ONE. ISSN 1932-6203 (Online) (doi:https://doi.org/10.1371/journal.pone.0182726)
• Milledge, J.; Heaven, S. (2017) Energy balance of biogas production from microalgae: Effect of harvesting method, multiple raceways, scale of plant and combined heat and power generation. J of Marine Sci and Eng, 5, 9.
• Onifade, T. B., Wandiga, S. O., Bello, I. A., Jekanyinfa, S. O. and Harvey, P. J.  (2017) Conversion of lignocellulose from palm (Elaeis guineensis) fruit fibre and physic (Jatropha curcas) nut shell into bio-oil. African Journal of Biotechnology, 16 (46). pp. 2167-2180. ISSN 1684-5315 (Online) (doi:https://doi.org/10.5897/AJB2017.16004)
• Aksnes, Dag Lorents, Holm, Poul, Bavinck, Maarten, Biermann, Frank, Donovaro, Roberto, Harvey, Patricia , Hynes, Stephen, Ingram, John, Kaiser, Matthias, Kaushik, Sachi, Krause, Gesche, Lapegue, Sylvie, Macdiarmid, Alison, Olsen, Yngvar, Quaas, Martin, Schmidt, Daniela, Sorgeloos, Patrick and St John, Michael (2017) Food from the Oceans - How can more food and biomass be obtained from the oceans in a way that does not deprive future generations of their benefits? Project Report. Science Advice for Policy by European Academies (SAPEA), Berlin. (doi:https://doi.org/10.26356/foodfromtheoceans)

2016

• Miest, Joanna J., Arndt, Carmen, Adamek, Mikolaj, Steinhagen, Dieter and Reusch, Thorsten B.H. (2016) Dietary b-glucan (MacroGard®) enhances survival of first feeding turbot (Scophthalmus maximus) larvae by altering immunity, metabolism and microbiota. Fish & Shellfish Immunology, 48. pp. 94-104. ISSN 1050-4648 (Print), 1095-9947 (Online) (doi:https://doi.org/10.1016/j.fsi.2015.11.013)
• Redden, Hilary, Milledge, John J. , Greenwell, H. Chris, Dyer, Philip W. and Harvey, Patricia  (2016) Changes in higher heating value and ash content of seaweed during ensiling. Journal of Applied Phycology, 29 (2). pp. 1037-1046. ISSN 0921-8971 (Print), 1573-5176 (Online) (doi:https://doi.org/10.1007/s10811-016-0975-4)
• Milledge, John J.  and Harvey, Patricia J.  (2016) Golden tides: Problem or golden opportunity? The valorisation of sargassum from beach inundations. Journal of Marine Science and Engineering, 4 (3):60. ISSN 2077-1312 (doi:https://doi.org/10.3390/jmse4030060)
• Xu, Yanan , Ibrahim, Iskander M. and Harvey, Patricia J.  (2016) The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30. Plant Physiology and Biochemistry, 106. pp. 305-315. ISSN 0981-9428 (doi:https://doi.org/10.1016/j.plaphy.2016.05.021)
• Milledge, John J.  and Harvey, Patricia J.  (2016) Potential process 'hurdles' in the use of macroalgae as feedstock for biofuel production in the British Isles. Journal of Chemical Technology & Biotechnology, 91 (8). pp. 2221-2234. ISSN 0268-2575 (Print), 1097-4660 (Online) (doi:https://doi.org/10.1002/jctb.5003)
• Huber, Christian, Preis, Martina, Harvey, Patricia J. , Grosse, Sylvia, Letzel, Thomas and Schröder, Peter (2016) Emerging pollutants and plants – Metabolic activation of diclofenac by peroxidases. Chemosphere, 146. pp. 435-441. ISSN 0045-6535 (Print), 1879-1298 (Online) (doi:https://doi.org/10.1016/j.chemosphere.2015.12.059)
• Milledge, John J.  and Harvey, Patricia J.  (2016) Ensilage and anaerobic digestion of Sargassum muticum.  Journal of Applied Phycology 28, 1573-5176. doi: 10.1007/s10811-016-0804-9

2015

• Milledge JJ, Nielsen B, and Bailey D (2015) High-value products from macroalgae: The potential uses of the invasive brown seaweed, Sargassum muticum. Reviews in Environmental Science and Bio/Technology, 1-22.
• Milledge JJ and Heaven S (2015) Energy Balance of Biogas Production from Microalgae: Development of an Energy and Mass Balance Model. Current Biotechnology 4, (4) 554-567.
• Xu, Y. , Milledge, John J. , Abubakar, A., Swamy, Rekha, Bailey, David and Harvey, Patricia  (2015) Effects of centrifugal stress on cell disruption and glycerol leakage from Dunaliella salina. Microalgae Biotechnology, 1 (1). pp. 20-27. ISSN 2300-3561 (Online) (doi:https://doi.org/10.1515/micbi-2015-0003)
• Balasubramaniyam, Anuluxshy and Harvey, Patricia J.  (2015) Changes in the abundance of sugars and sugar-like compounds in tall fescue (Festuca arundinacea) due to growth in naphthalene-treated sand. Environmental Science and Pollution Research, 22 (8). pp. 5817-5830. ISSN 0944-1344 (Print), 1614-7499 (Online) (doi:https://doi.org/10.1007/s11356-014-3812-9)
• Milledge, John J. , Staple, Alan and Harvey, Patricia J.  (2015) Slow pyrolysis as a method for the destruction of Japanese wireweed, Sargassum muticum. Environment and Natural Resources Research, 5 (1). pp. 28-37. ISSN 1927-0488 (Print), 1927-0496 (Online) (doi:https://doi.org/10.5539/enrr.v5n1p28)
• Balasubramaniyam, Anuluxshy, Chapman, Mark M. and Harvey, Patricia J.  (2015) Responses of tall fescue (Festuca arundinacea) to growth in naphthalene-contaminated sand: xenobiotic stress versus water stress. Environmental Science and Pollution Research, 22 (10). pp. 7495-7507. ISSN 0944-1344 (Print), 1614-7499 (Online) (doi:https://doi.org/10.1007/s11356-015-4084-8)

2014

• Milledge, John J. , Smith, Benjamin, Dyer, Philip W. and Harvey, Patricia  (2014) Macroalgae-derived biofuel: A review of methods of energy extraction from seaweed biomass. Energies, 7 (11). pp. 7194-7222. ISSN 1996-1073 (doi:https://doi.org/10.3390/en7117194)
• Harvey, Patricia , Bailey, David, Ben-Amotz, Ami, Verdelho, Vitor, Harris, Guy, Rooke, David, Hoekstra, Herre, Goacher, Paul, Crespi, Joao, Reinhardt, Guido, Martinelli, Laura, Schroder, Declan, Pipe, Richard, Igl-Schmid, Nadine, Kokossis, Antonis and Perrson, Karin (2014) The CO2 microalgae biorefinery: high value products and biofuels using halophilic microalgae in the “D-Factory”. New Biotechnology, 31 (Supp.). S14-S15. ISSN 1871-6784 (doi:https://doi.org/10.1016/j.nbt.2014.05.1644)
• Balasubramaniyam, Anuluxshy and Harvey, Patricia J.  (2014) Scanning electron microscopic investigations of root structural modifications arising from growth in crude oil-contaminated sand. Environmental Science and Pollution Research, 21 (22). pp. 12651-12661. ISSN 0944-1344 (Print), 1614-7499 (Online) (doi:https://doi.org/10.1007/s11356-014-3138-7)
• Falco, Alberto, Miest, Joanna Junack, Pionnier, Alberto, Pietretti, Danilo, Forlenza, Maria, Wiegertjes, Geert F. and Hoole, Dave (2014) b-Glucan-supplemented diets increase poly(I:C)-induced gene expression of Mx, possibly via Tlr3-mediated recognition mechanism in common carp (Cyprinus carpio). Fish & Shellfish Immunology, 36 (2). pp. 494-502. ISSN 1050-4648 (Print), 1095-9947 (Online) (doi:https://doi.org/10.1016/j.fsi.2013.12.005)

BOOKS

• Milledge, J.J.; Maneein, S. (2020) Chapter 6 - Storage of seaweed for biofuel production: Ensilage. In Sustainable Seaweed Technologies, Torres, M.D., Kraan, S., Dominguez, H., Eds. Elsevier: 155-167.
• Milledge, John J. , Thompson, Elinor P. , Sauvetre, Andre, Schroeder, Pater and Harvey, Patricia  (2019) Novel developments in biological technologies for wastewater processing. In: Galanakis, Charis M. and Agrafioti, Evita, (eds.) Sustainable Water and Wastewater Processing. Elsevier, pp. 239-278. ISBN 978-0128161708 (doi:https://doi.org/10.1016/B978-0-12-816170-8.00008-9)
• Milledge, John J.  and Harvey, Patricia J.  (2018) Anaerobic digestion and gasification of seaweed. In: Rampelotto, Pabulo H. and Trincone, Antonio, (eds.) Grand Challenges in Marine Biotechnology. Grand Challenges in Biology and Biotechnology . Springer International Publishing, Cham, pp. 237-258. ISBN 978-3319690742 (doi:https://doi.org/10.1007/978-3-319-69075-9_7)
• Psycha, Melina, Pyrgakis, Kostantinos, Harvey, Patricia J. , Ben-Amotz, Ami, Cowan, A. Keith and Kokossis, Antonis C. (2014) Design analysis of integrated microalgae biorefineries. In: Eden, Mario R., Siirola, John D. and Towler, Gavin P., (eds.) Proceedings of the 8th International Conference on Foundations of Computer-Aided Process Design. Computer Aided Chemical Engineering (34). Elsevier B.V., Amsterdam, The Netherlands, pp. 591-596. ISBN 978-0-444-63433-7 ISSN 15707946 (doi:https://doi.org/10.1016/B978-0-444-63433-7.50083-3)
• M. E. Nielsen, J. J. Miest, and J. G. Schmidt (2013) “The Immune System” In: Eel Physiology. ed. by Trischitta, F., Takei, Y. and Sebert, P. CRC Press, Boca Raton, Fl., USA, p. 298. ISBN 978-1-4665-9827-0

PATENTS

• P.J. Harvey, Y. Xu, Production of Dunaliella, Patent WO/2019/097219, Priority filing date 14.11.2017, 2019.
• D. Schroeder, P.J. Harvey, Y. Xu, Algal Strains Patent WO/2018/141978 Priority filing date 03.02.2017, 2018