Professor Maruthi M N Gowda
Professor of Molecular Plant Pathology
BSc, MSc, PhD
Agriculture, Health & Environment Department
Natural Resources Institute
Professor Maruthi Gowda is a molecular plant virologist and vector entomologist with 20 years of research experience in plant-virus-insect interactions, especially those involving geminiviruses, potyviruses and their whitefly vector, Bemisia tabaci, infecting cassava, vegetables and other staple food crops in the tropics.
He began his research career in India in 1996 when he identified sources of resistance to the economically important tomato leaf curl disease. He then moved to the Natural Resources Institute at the University of Greenwich in 1998 to undertake his PhD on cassava mosaic disease, which has caused devastating losses, famine and food shortages to subsistent farmers in Africa. Following completion of his PhD in 2001, Professor Gowda joined the university as a post-doctoral fellow. He was promoted to research fellow and subsequently senior research fellow.
Currently, as a Professor of Molecular Plant Pathology, Professor Gowda leads a core team of researchers (six PhDs, and one research fellow) investigating the reasons for the recent outbreak of cassava brown streak disease (CBSD) in the eastern and southern African region. He employs multi-disciplinary research, from field epidemiology to molecular biology and tissue culture to functional genomics, to better understand plant-virus-vector relationships and mechanisms of disease resistance to enable developing improved disease control strategies. He was the first to identify whitefly B. tabaci as the natural vector of cassava brown streak viruses (CBSVs). He has also developed robust low-cost diagnostic tests for CBSVs and cassava mosaic viruses, which are adapted in several African laboratories.
Through a Bill and Melinda Gates Foundation funded multi-partner project (5CP, 2012–16), Professor Gowda was able to develop a highly sensitive virus-indexing programme for cleaning virus-infected cassava landraces and contributed to the conservation of germplasm. Through the Grand Challenges Explorations grant (2011–13), also from the Gates Foundation to work on an innovative idea for controlling whiteflies using endosymbiotic bacteria. Other sources of funding for his research comes from European Union (partner in CassavaGMarkets project, 2012–16), African Union (leading LimitCBSD project, 2012–15) and other sources.
He is an active member in the plant virology community, both in the UK and overseas. In the UK, he is the chair of the Plant Virology Committee of the Association of Applied Biologists, responsible for organising international symposia every 18 months. Dr Gowda co-hosted the 2007 AAB symposium and will be hosting the next meeting 7-9 September 2016 at the University of Greenwich.
Professor Gowda is passionate about using advanced molecular technologies to mitigate pest and disease problems in the tropics, especially those of cassava in Africa. He is currently interested in gaining greater understanding of cassava brown streak disease, which is epidemic in eastern Africa, its negative effects on the poor and devising ways to control the disease. He is working to identify natural sources of resistance to viruses and their insect vectors using high throughput sequencing (RNA-Seq) to identify, characterise, map and understand the mechanism of resistance genes with the aim of developing durable resistance to the disease.
Another complementary and exciting area of his research includes using endosymbiotic bacteria as bio-control agent against whitefly, the vector of cassava viruses. Professor Gowda's team is identifying certain strains of bacteria that can reduce reproduction and increase mortality of whiteflies. If successful, this self-perpetuating bio-control strategy is expected to reduce cassava whitefly numbers significantly, which has great potential to achieve marked decrease in cassava disease incidences. The knowledge generated can also contribute to the greater understanding of how vector-borne diseases affect human beings.
Professor Gowda's aim is to take holistic approaches to find solutions for cassava pest and disease problems for increased food security of the poor in eastern Africa.
Current and Previous Funded Research Projects
Whitefly control using Wolbachia, 2012–2013.
This highly innovative Gates Foundation-funded project to control the agricultural pest whiteflies using endosymbiotic bacteria called Wolbachia was led by Professor Gowda. This was a pilot project which ran for 18 months, but Phase II was not funded. Research, however, continued on this interesting topic through a University of Greenwich PhD studentship. Wolbachia infects over 75% of the earth's insect population, and are shown to negatively affect the lifecycles of certain insect species and can also reduce pathogen transmission by them. Certain strains of Wolbachia are therefore used as potential bio-control agents for controlling diseases of medical importance (eg. dengue viruses) transmitted by mosquitoes. Since whiteflies are naturally infected with Wolbachia, the researchers are investigating whether a similar strategy can be employed to reduce the high whitefly populations on cassava in Africa. If successful, this strategy has huge potential for controlling whiteflies and the viruses they transmit since it comes at no-cost to the farmers, is self-perpetuating and can be implemented together with existing control strategies. For this reason, it can be part of an IPM package for the sustainable control of both whiteflies and virus diseases. The most exciting aspect of this research, apart from controlling cassava virus diseases, is the generation of fundamental knowledge that can contribute to better understanding of vector-transmitted pathogens of medical importance. Total value of the project USD 100K.
Limiting the impact of cassava brown streak disease on smallholders, women and the cassava value chain (LimitCBSD). 2012–2015.
Professor Gowda is leading this African Union-funded multi-partner project to mitigate the impact of CBSD in Kenya, Tanzania and Malawi. The main aim of the project is to better understand the recent disease outbreaks in east Africa and devise effective control strategies. The researchers will first carry out socio-economic research to understand the impact of the disease on the poor, in particular on woman farmers who are the main workforce for cassava cultivation. They will then screen new cassava lines for viruses to identify resistant varieties and use state-of-the-art next generation sequencing for identifying genes that are contributing to resistance. This information will be used for developing disease resistance molecular markers for integration into breeding programmes. The successful implementation of this project will significantly improve the capacity of local partners in controlling the disease. Total value of the project Euros 620K.
New cassava varieties and clean seed to combat CMD and CBSD project (5CP), 2012–2016.
Professor Gowda is a partner in this multi-partner project, led by IITA and funded by the Gates Foundation. The project, implemented in five eastern African countries, aims to identify CBSD resistance in 25 elite cassava lines in five target countries and develop a commercial seed system for cassava. Professor Gowda's role is to develop a virus indexing programme for cleaning 25 lines from virus infection using a combination of tissue culture, thermotherapy and chemotherapy, and virus diagnosis. As part of this project, the researchers have successfully developed a virus indexing programme for the African cassava viruses and so far cured over 40 cassava lines from virus infections. Methodologies developed at NRI have wider applications beyond the scope of 5CP as they will contribute in preventing virus-susceptible cassava varieties from extinction and the spread of virus-infected plants to new regions. The NRI component of this project is successfully delivered now. NRI contract value in the project USD 222K.
Improving the Livelihoods of Smallholder Cassava Farmers Through Better Access to Growth Markets (CassavaGMarkets), 2012-2016.
This multi partner project, led by NRI, funded by the European Commission is aiming to improving the livelihoods of smallholder cassava farmers through better access to growth markets (CassavaGmarkets). Dr Gowda is leading one of the seven work packages of this project, to better understand the epidemiology of CBSD in the disease endemic areas of southern Tanzania. Research will be carried out to identify disease resistant varieties for this region and the next generation sequencing will be used for identifying CBSD resistant genes. Total value of the project Euros 3.5 million.
Building Local Capacity for Surveillance Diagnosis, Characterisation and Control of Cassava Viruses in Northern Nigeria, 2014-2017
Professor Gowda is a Co-PI in this PEARL project led by the Kebbi State University of Science and Technology in Nigeria. Main aim of the project is to build human and physical capacity through training and setting up a virus diagnostic laboratory that is capable of carrying out surveillance, diagnosis and characterization of viruses affecting cassava in northern Nigeria. University researchers, postgraduate students, extension workers and quarantine officers will be trained to identify cassava diseases in the field and also using diagnostic molecular techniques in the lab. Field surveys will be conducted in the nine states of the northern Nigeria to determine the prevalence of CMD including the recently introduced severe strain East African cassava mosaic virus-Uganda (EACMV-Ug) in the region. The EACMV-Ug resistance levels of Nigerian cassava varieties and local land races grown by farmers will be evaluated in field trials. As pre-emptive preparedness, the Nigerian cassava germplasm will be tested for resistance to another important viral disease, the cassava brown streak disease (CBSD) in East Africa through collaborations. CBSD is currently restricted to Eastern Africa but poses greatest threat to cassava production in West Africa through potential inadvertent introductions. Local quarantine officials will be trained and awareness will be created on the potential dangers of introducing CBSD to Nigeria. Added value of this proposal is to build capacity for cassava disease surveillance in northern Nigeria, an area that has long been neglected but equally important for food security and economic development of the farmers. The long term benefits of this investment can be to use the laboratory set up as a strategic point for disease surveillance in West Africa for cassava as well as other important food crops such as yams and legumes. NRI contract value £166K.
African cassava whitefly project: outbreak causes and sustainable solutions project, 2014-2018.
Professor Gowda is a Co-PI in this mega project http://cassavawhitefly.org/ led by NRI. He is contributing to three key areas of research and development; to identify natural sources of resistance to African cassava whitefly species in local and exotic cassava lines, and to investigate the role of endosymbionts on whitefly biology, virus transmission and population ecology to understand the phenomenon of whitefly super abundance in African countries.
Responsibilities within the University
Responsible for the implementation and delivery of externally funded projects, supervising PhDs and MSc students and junior members of staff, part responsible for smooth running of molecular, insectary and glasshouse facilities, maintaining H&S records and their implementation.
- Hillocks R, Maruthi MN, Kulembeka H, Jeremiah S, Alacho F, Masinde E, Ogendo J, Arama P, Mulwa R, Mkamilo G, Kimata B, Mwakanyamale D, Mhone A, Benesi I, 2015. Disparity between leaf and root symptoms and economic losses associated with cassava brown streak disease in four countries in eastern Africa. Journal of Phytopathology. On-line early, doi:10.1111/jph.12430
- Mohammed IU, Ghosh S, Maruthi MN, 2015. Host and virus factors affecting reversion to cassava brown streak disease in cassava. Plant Pathology. Doi: 10.1111/ppa.12458
- Hillocks RJ and Maruthi MN, 2015. Post-harvest impact of cassava brown streak disease in four countries in eastern Africa. Food Chain 5:116-122.
- Ghosh S, Bouvaine S, Maruthi MN. 2015. Prevalence and genetic diversity of endosymbiotic bacteria infecting cassava whiteflies in Africa. BMC Microbiology 15:93 DOI 10.1186/s12866-015-0425-5
- Collins C, Maruthi MN, Jahn CE. 2015. CYCD3 D-type cyclins regulate cambial cell proliferation and secondary growth in Arabidopsis. Journal of Experimental Botany. doi:10.1093/jxb/erv218
- Maruthi MN, Bouvaine S, Tufan HA, Mohammed IU, Hillocks RJ (2014) Transcriptional Response of Virus-Infected Cassava and Identification of Putative Sources of Resistance for Cassava Brown Streak Disease. PLoS ONE 9(5): e96642. doi:10.1371/journal.pone.0096642.
- James Legg, Eklou Attiogbevi Somado, Ian Barker, Larry Beach, Hernan Ceballos, Willmer Cuellar, Warid Elkhoury, Dan Gerling, Jan Helsen, Clair Hershey, Andy Jarvis, Peter Kulakow, Lava Kumar, Jim Lorenzen, John Lynam, Matthew McMahon, Gowda Maruthi, Doug Miano, Kiddo Mtunda, Pheneas Natwuruhunga, Emmanuel Okogbenin, Phemba Pezo, Eugene Terry, Graham Thiele, Mike Thresh, Jonathan Wadsworth, Steve Walsh, Stephan Winter, Joe Tohme, Claude Fauquet, 2014. A global alliance declaring war on cassava viruses in Africa. Food Security 6:231–248. DOI 10.1007/s12571-014-0340-x.
- Al-Kuwaiti N, Otto B, Collins C, Seal S, Maruthi MN, 2013. Molecular characterisation and first complete genome sequence of Tomato yellow leaf curl virus (TYLCV) infecting tomato in Iraq. New Disease Reports 27, 17.
- Abarshi, M.M., Mohammed, I.U., Jeremiah, S.C., Legg, J.P., Kumar, P. Lava, Hillocks, R.J. and Maruthi, M.N. (2012) Multiplex RT-PCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa. Journal of Virological Methods, 179 (1). pp. 176-184. ISSN 0166-0934 (doi:10.1016/j.jviromet.2011.10.020)
- Mohammed, I.U., Abarshi, M.M., Muli, B., Hillocks, R.J. and Maruthi, M.N. (2012) The symptom and genetic diversity of cassava brown streak viruses infecting cassava in East Africa. Advances in Virology, 2012. ISSN 1687-8639 (Print), 1687-8647 (Online) (doi:10.1155/2012/795697)
- Mbanzibwa, D.R., Tian, Y.P., Tugume, A.K., Patil, B.L., Yadav, J.S., Bagewadi, B., Abarshi, M.M., Alicai, T., Changadeya, W., Mkumbira, J., Muli, M.B., Mukasa, S.B., Tairo, F., Baguma, Y., Kyamanywa, S., Kullaya, A., Maruthi, M.N., Fauquet, C.M. and Valkonen, J.P.T. (2011) Evolution of cassava brown streak disease associated viruses. Journal of General Virology, 92 (4). pp. 974-987. ISSN 0022-1317 (Print), 1465-2099 (Online) (doi:10.1099/vir.0.026922-0)
- Legg, J.P., Jeremiah, S.C., Obiero, H.M., Maruthi, M.N., Ndyetabula, I., Okao-Okuja, G., Bouwmeester, H., Bigirimana, S., Tata-Hangy, W., Gashaka, G., Mkamilo, G., Alicai, T. and Lava Kumar, P. (2011) Comparing the regional epidemiology of the cassava mosaic and cassava brown streak virus pandemics in Africa. Virus Research, 159 (2). pp. 161-170. ISSN 0168-1702 (doi:10.1016/j.virusres.2011.04.018)
- Patil, Basavaprabu L., Ogwok, Emmanuel, Wagaba, Henry, Mohammed, Ibrahim U., Yadav, Jitender S., Bagewadi, Basavaraj, Taylor, Nigel J., Kreuze, Jan F., Maruthi, M.N., Alicai, Titus and Fauquet, Claude M. (2011) RNAi-mediated resistance to diverse isolates belonging to two virus species involved in Cassava brown streak disease. Molecular Plant Pathology, 12 (1). pp. 31-41. ISSN 1464-6722 (Print), 1364-3703 (Online) (doi:10.1111/j.1364-3703.2010.00650.x)
- Abarshi, M.M., Mohammed, I.U., Wasswa, P., Hillocks, R.J., Holt, J., Legg, J.P., Seal, S.E. and Maruthi, M.N. (2010) Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus. Journal of Virological Methods, 163 (2). pp. 353-359. ISSN 0166-0934 (doi:10.1016/j.jviromet.2009.10.023)
- Ahmed, Muhammad Z., Ren, Shun-Xiang, Mandour, Nasser S., Maruthi, M.N., Naveed, Muhammad and Qiu, Bao-Li (2010) Phylogenetic analysis of Bemisia tabaci (Hemiptera: Aleyrodidae) populations from cotton plants in Pakistan, China, and Egypt. Journal of Pest Science, 83 (2). pp. 135-141. ISSN 1612-4758 (Print) 1612-4766 (Online) (doi:10.1007/s10340-009-0279-4)
- Lava Kumar, P., Ayodele, M., Oben, T.T., Mahungu, N.M., Beed, F., Coyne, Daniel L., Londa, L., Mutunda, M.P., Kiala, D. and Maruthi, M.N. (2009) First report of Banana bunchy top virus in banana and plantain (Musa spp.) in Angola. Plant Pathology, 58 (2). p. 402. ISSN 0032-0862 (Print), 1365-3059 (Online) (doi:10.1111/j.1365-3059.2008.01974.x)
- Shankarappa, K.S., Sriharsha, K.T., Aswathanarayana, D.S., Prameela, H.A., Kulkarni, R.S., Muniyappa, V., Rao, A.M. and Maruthi, M.N. (2008) Development of tomato hybrids resistant to tomato leaf curl virus disease in South India. Euphytica, 164 (2). pp. 531-539. ISSN ISSN: 0014-2336 (print version) ISSN: 1573-5060 (electronic version) (doi:10.1007/s10681-008-9750-1)
- Shankarappa, K.S., Rangaswamy, K.T., Aswathanarayana, D.S., Rekha, A.R., Raghavendra, N., Lakshminarayana Reddy, C.N., Chancellor, Tim and Maruthi, M.N. (2007) Development of silverleaf assay, protein and nucleic acid-based diagnostic techniques for the quick and reliable detection and monitoring of biotype B of the whitefly, Bemisia tabaci (Gennadius). Bulletin of Entomological Research, 97 (5). pp. 503-513. ISSN 0007-4853 (doi:10.1017/S0007485307005251)
- Maruthi, M.N., Rekha, A.R. and Muniyappa, V. (2007) Pumpkin yellow vein mosaic disease is caused by two distinct begomoviruses: complete viral sequences and comparative transmission by an indigenous Bemisia tabaci and the introduced B-biotype. EPPO Bulletin, 37 (2). pp. 412-419. ISSN 0250-8052 (doi:10.1111/j.1365-2338.2007.01127.x)
- Maruthi, M.N., Rekha, A.R., Mirza, S.H., Alam, S.N. and Colvin, John (2007) PCR-based detection and partial genome sequencing indicate high genetic diversity in Bangladeshi begomoviruses and their whitefly vector, Bemisia tabaci. Virus Genes, 34 (3). pp. 373-385. ISSN 0920-8569 (doi:10.1007/s11262-006-0027-2)
- Maruthi, M.N., Rekha, A.R., Sseruwagi, P. and Hillocks, Rory (2007) Mitochondrial DNA variability and development of a PCR diagnostic test for populations of the whitefly Bemisia afer (Priesner and Hosny). Molecular Biotechnology, 35 (1). pp. 31-40. ISSN 1073-6085 (doi:10.1385/MB:35:1:31)
- Aswatha Narayana, D.S., Rangaswamy, K.T., Shankarappa, K.S., Maruthi, M.N., Lakshminarayana Reddy, C.N., Rekha, A.R. and Keshava Murthy, K.V. (2007) Distinct begomoviruses closely related to cassava mosaic viruses cause Indian Jatropha mosaic disease. International Journal of Virology, 3 (1). pp. 1-11. ISSN 1816-4900 (doi:10.3923/ijv.2007.1.11)
- Alicai, T., Omongo, C.A., Maruthi, M.N., Hillocks, Rory, Baguma, Y., Kawuki, R., Bua, A., Otim-Nape, G.W. and Colvin, John (2007) Re-emergence of Cassava Brown Streak Disease in Uganda. Plant Disease, 91 (1). pp. 24-29. ISSN 0191-2917 (doi:10.1094/PD-91-0024)
Recent Conference Presentations
- Maruthi M.N. 2015. Transcriptional response of virus-infected cassava and identifying virus resistance genes and markers for cassava brown streak disease. The BSPP Presidential meeting – The impact of Plant Pathology on Everyday Lives, 13-15 September 2015, Bristol, UK.
- Maruthi M.N. 2014, Recent progress on CBSD research and control. International cassava workshop in La Réunion – Surveillance and control of cassava viral and bacterial diseases on the African continent La Réunion, St Pierre, June 10-13, 2014.
- Maruthi, M. N. (2013) Cassava brown streak virus disease – understanding a difficult disease for providing effective control. International Conference on Tropical Roots and Tubers for Sustainable Livelihood Under Changing Agro-Climate, 9-12 July 2013, Thiruvanthapuram, India.
- Maruthi, M. N., Laniran, P., Ghosh, S. (2013) Wolbachia and the biological control of cassava whiteflies. First International Whitefly Symposium, 20-24 May 2013, Kolymbari, Crete, Greece.
- Maruthi, M.N., Mohammed, I.U., Tufan, H.A., Hillocks R.J. (2013) Identifying cassava resistant varieties and resistance genes for cassava brown streak disease. 12th International Symposium on Plant Virus Epidemiology 28 January – 1 February 2013. Arusha, Tanzania.
- Maruthi, M.N., Abarshi M.M., Legg, J.P., Kumar, P.L., Hillocks, R.J. (2013) Multiplex RT-PCR assays for the simultaneous detection of cassava mosaic virus and cassava brown streak viruses. 12th International Symposium on Plant Virus Epidemiology 28 January – 1 February 2013. Arusha, Tanzania.
- Maruthi, M.N., Mohammed, I.U., Hillocks, R.J. (2013) Exploiting reversion and tissue culture techniques for eliminating cassava brown streak viruses from infected cassava plants. 12th International Symposium on Plant Virus Epidemiology 28 January – 1 February 2013. Arusha, Tanzania.
- Maruthi, M.N., Jeremiah, S., Mohammed, I.U., Legg, J.P. (2013) Virus-vector transmission relationships for cassava brown streak viruses and the whitefly vector, Bemisia tabaci. 12th International Symposium on Plant Virus Epidemiology 28 January – 1 February 2013. Arusha, Tanzania.
- Abarshi, M.M., Mohammed, I.U., Legg J.P., Kumar P. L., Maruthi, M.N. (2012) Multiplex RT-PCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa. International Conference on Advances in Plant Virology, 28-30 March 2012, Dublin, Ireland.
- Mohammed, I.U., Abarshi, M.M., Hillocks, R.J., Maruthi, M.N. (2012) Virus-host interactions in Cassava brown streak disease-pathosystem. International Conference on Advances in Plant Virology, 28-30 March 2012, Dublin, Ireland.
- Mohammed, I. U., Abarshi, M. M., Hillocks, R. J., Maruthi, M. N. (2012) Developing methods to eliminate Cassava brown streak viruses from infected cassava varieties. International Conference on Advances in Plant Virology, 28-30 March 2012, Dublin, Ireland.
- Al_Kuwaity, N., Seal, S., Maruthi, M.N. (2012) Molecular characterization of plant viruses infecting potato and vegetables in Iraq. International Conference on Advances in Plant Virology, 28-30 March 2012, Dublin, Ireland.
- Editor of VirusDisease – 2010 - present
- Convenor of the AAB Virology committee meetings, panel member, 2007-present
- Chair of the AAB Virology committee – 2015- present
- Biological and Genetic Modification Safety Committee, UoG, 2008-present
- Association of Applied Biologists, 2001- present
- European and International Whitefly Studies Network (EWSN), 2000-present
- ssDNA virus and Geminivirus study groups, 2001 - present
- Working Group on Legume and Vegetable Viruses (IWGLV), 2005-present
- British Society for Plant Pathology, 1999-2000
- 'Promising Researcher Award' 2004-05 funded by HEFCE administered by UoG
Professor Maruthi Gowda
Natural Resources Institute
University of Greenwich
Central Avenue, Chatham Maritime, Kent ME4 4TB, United Kingdom.
Tel: +44 (0) 1634 883957